Khajavi, Saeed (2014) Procedure optimization of protein hydrolysis with highest antioxidant activity from Shizothorax (Shizothorax Zarudnyi) wastes using response surface methodology. Masters thesis, University of Zabol.
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Abstract
Oxidation plays the important role in diseases and reduces quality of fats and oil in food products during processing and storage. The fish processing industry produces more than 60% by-products as waste, which includes skin, head, viscera, liver, and ... . These by-product wastes contain good amount of protein rich material that are normally processed into low market-value products, such as animal feed, fish meal and fertilizer. But we can use from these wastes on Products with higher added value such as fish protein hydro lysate white Functional properties and antioxidants activity. In this study Shizothorax Zarudnyi wastes were hydrolyzed by pepsin proteases. DPPH radical scavenging power was used to evaluate anti-oxidative activity of hydro lysates. One variable at a time and response surface methodology (RSM) were employed to optimize hydrolysis conditions, including enzyme concentration, substrate concentration, reaction temperature, and hydrolysis time. Central composition rotatable design (CCRD) was used for this study. The optimum conditions obtained in RSM were as follows: enzyme concentration of 3%, substrate concentration of 39.89%, temperature of 35.17 ◦C and time of 38.6 hours, under which, DPPH radical scavenging activity of 61.85% was found.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Shizothorax Zarudnyi, Protein hydro lysate, Antioxidant activity, Response surface methodology |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 23 May 2016 08:19 |
| Last Modified: | 23 May 2016 08:19 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/642 |
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