Effect of Nisin and Cuminum cyminum essential oil on Staphylococcus aureus in Hypophthalmichthys molitrix minced meat

Faridi, M. (2015) Effect of Nisin and Cuminum cyminum essential oil on Staphylococcus aureus in Hypophthalmichthys molitrix minced meat. Masters thesis, University of Zabol.

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Abstract

Staphylococcus aurous is a pathogen of food that is a serious hazard for human food industry. This study aimed to evaluate the chemical composition of Cumin (Cuminum cyminum) essential oil and its antibacterial activity in inhibiting of S. Aurous bacteria (Staphylococcus aurous) alone and in combination with Nisin in minced meat silver carp was investigated. Extraction of essential oil was performed using steam distillation method by Clevenger apparatus and its chemical composition was analyzed by the Gas chromatography-mass spectrometry device. Cumin aldehyde with 27/20% was the main component in cumin essential oil. The effects of different concentrations of cumin essential oil (0, 0/015, 0/03, 0/06, 0/09 and 0/1% ), Nisin (0, 2/5, 3/5 and 5 µg/g) and a combination of varying amounts of tow materials on growing behavior of the bacteria at 4° c temperature over 21days were investigated. The results showed that the bacterial growth at various concentrations of cumin essential oil and Nisin has stopped on 12 and 3 day (p<0.05) respectively. Low concentrations of cumin essential (0/015 and 0/03%) did not have significant differences to inhibit bacterial growth (p>0/05). Suitable inhibition effects for Staphylococcus aurous growing was observed at concentrations of 0/09 and 0/1% of cumin essential oil (p<0/05). Nisin, at all used levels, could stop bacterial growth in different days of keeping period (p<0/05). Combination treatments were effective in reducing the rate of bacterial growing, but treatment oil 1/0% to 2.5 micrograms per gram Nisin could stop the growth of bacteria after 6 days (p<0.05).

Item Type: Thesis (Masters)
Uncontrolled Keywords: Silver carp (Hypophthalmichthys molitrix), Staphylococcus aurous, cumin (Cuminum cyminum), essential oil, minced meat
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Depositing User: admin admin1 admin2
Date Deposited: 24 Apr 2016 05:41
Last Modified: 24 Apr 2016 05:41
URI: http://eprints.uoz.ac.ir/id/eprint/494

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