Effect of Diluents’ solutions on the fertilization of Snow trout (Schizothorax Zarudnyi)

Alizadeh Sargazi, Abbas (2013) Effect of Diluents’ solutions on the fertilization of Snow trout (Schizothorax Zarudnyi). Masters thesis, University of Zabol.

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Abstract

The Snow Trout (Schizothorax Zarudnyi) is an endangered species of endemic fish in Hamoun Lake, Sistan, Iran. Natural fish population have declined during the last several years because of the continuation of recent drought, shortage of food sources and existence of non-endemic species. So, the possibility of improvement of breeding methods with using of diluents solutions in order to increase of fertilization, eyed egg and hatching was studied. For this purpose eight female broods with average weight and length 1545/62±295/43 gr and 54/37±3/85 cm respectively and sixteen male brood with average weight and length 623/08±180/69 gr and 38/91±5/40 cm were selected respectively. Then mixed sperms and egg without any Coelumic fluid was fertilized under four treatment groups including: Treatment Number one: 5/54 gr NaCl, 2/422 gr Triss, 3/57 gr Glycin, regulated in pH=8/4 and solution in 1 lit distilled water. Treatment Number two: 7/305 gr NaCl, 0/735 gr CaCl2.2H2o, regulated in pH=8/4 and solution in 1 lit distilled water. Treatment Number three: Coelumic fluid that was separated from egg by using net. Treatment Number four: distilled water as control treatment. The results showed that motility duration of sperm in 1th, 2th, 3th and 4th treatments was 65/25±7/36, 30/8±12/83, 73/33±2/117, 40/00±1/61 sec. respectively, and the duration of forward movement was 6/25±4/71, 15/00±12/63, 52/33±1/90, 34/66±2/51 sec. respectively and eyed eggs percentage was 16/82±6/42, 53/00±4/66, 82/50±3/84, 73/33±2/08 respectively and hatching percentage was 55/05±5/42, 71/43±081, 49/60±6/25, 37/72±4/32. The study of Spermatology parameters and the percentage of eyed egg and hatching showed that that were a significant difference between eyed egg percentage and hatching in the number one and two (p،ـ0/05). So treatment number two is an effective diluents solution for improving breeding of this fish.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Schizothorax Zarudnyi, diluents solutions, Sperm motility, Sistan
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Depositing User: admin admin1 admin2
Date Deposited: 19 Apr 2016 05:13
Last Modified: 19 Apr 2016 05:13
URI: http://eprints.uoz.ac.ir/id/eprint/378

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