Kord Sahlabadi, M. (2011) Medicinal plant (Nepeta binaludensis Jamzad) tissue culture and nepetalactone variations of essential oil in vivo and in vitro. Masters thesis, University of Zabol.
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Abstract
Nepeta binaludensis Jamzad is an important medicinal species of Lamiaceae family. It is an endemic species with limited habitat in the highlands of northeastern Binalude Mountains, Iran. The main Essential oils components the plant is secondary metabolites nepetalactone and 1, 8 Cineole. This study was conducted to investigate the effect of in vitro conditions on N. binadulens nepetalactone content and cis-trans type. The experiment was conducted as a factorial experiment based on a completely randomized design with four replications. Callus induction was performed using gradient levels of 2, 4-D (0, 0.5, 1, 1.5, 2, and 2.5 mg.l-1) and BAP (0, 0.5, 1 and 1.5 mg.l-1). GC/MS analysis was done to compare the nepetalactone content among in vitro and in vivo conditions. The results suggest that nepatalactone synthesis showed an increase in 16 growth regulator combinations compared to hormone-free medium and in vivo conditions. The highest callus induction was obtained in 2mg/l 2, 4-D+1mg/l BAP. The results show that in vitro techniques are feasible method for nepetalactone synthesis.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Nepeta binaludensis Jamzad, Tissue culture, Essential oils, Trans- cis Nepetalactone, 2,4-D, BAP3 |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 06 Nov 2016 06:27 |
| Last Modified: | 06 Nov 2016 06:27 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/970 |
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