Cheraghi, M. H. (2009) Identification and distribution of Gaeumannomyces graminis varieties using morphological and molecular methods in wheat fields in Fars province. Masters thesis, University of Zabol.
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Abstract
During growing seasons of 1386-87 wheat and weeds infected to take-all were collected from infected samples, 28 isolated from Gaeumannomyces graminis var. tritici (Ggt) were isolated and purified. Pathogenicity test of the isolates was conducted on wheat, rye and oat. The isolates showed the disease symptom on wheat, but not on oat. The disease symptom and signs was highly significant in wheat and rye compared with oat. After almost 7-8 weeks of pathogenicity, a lot of perithecia were observed on crown and roots. Conventional diagnosis of the pathogen is time-consuming, laborious and often inconclusive. Otherwise complexity of the soil microorganisms enhances difficulty in diagnosis of wheat take-all For example the Gaeumannomycs- Phialophora Complex and the non-pathogen Gaeumannomyces and Phialophora species found on cereal roots are troublemaker. Therefore, the objective of this study was to develop a rapid, simple and species method for differentiation of Gaeumannomyces graminis variety using PCR and molecular-based techniques. Simple hyphopodia which grows on lower sheets of wheat and average length of ascosporic have shown that all of the isolates belong to Ggt. The isolated Ggts were divided into two subdivisions, R (infected rye) and N (non infected rye). Reaction of the 28 isolates were positive in PCR technique by a pair of primer (NS5: GGT-RP).The primer was designed from the 18S rDNA region of wheat. The morphological and molecular results showed that all of the isolates belonged to Ggt. Enzyme digestion of some isolares with four enzyme AluI, CfoI, HinfI and RsaI showed no genetic diversity.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Gaeumannomyces graminis, Identification, Diversity Morphological and Molecular |
| Subjects: | Q Science > QK Botany |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Oct 2016 08:43 |
| Last Modified: | 25 Oct 2016 08:43 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/907 |
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