khodadadi, Fatemeh (2010) Identification and determination of genetic diversity aflatoxin producing Aspergillus spp. in pistachio nuts using RAPD. Masters thesis, University of Zabol.
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Abstract
Pistachio nut is one of the most important agriculture crops in Iran. The product is attacked by aflatoxigenic fungi A. flavus and A. parasiticus. The toxin produced in the nuts by the fungi is detrimental to human and animal health feeding from contaminated nuts. The objective of the project was to detect and determine the genetic of diversity of the aflatoxigenic isolates in the stored pistachio nuts. The contaminated pistachio nuts were collected from the stored nuts in Kerman province. The isolates of fungal species were separated, purified and then identified based on morphological characteristics using Aspergillus key. The HPLC was employed to determine the type and rate of aflatoxins produced by the fungi in nuts. The PCR method by using the primers designed from AFIR gene utilized to identify aflatoxigenic isolates. The RAPD technique by five 20-mer primers was used to genetically identify the aflatoxigenic fungi. As was expected a 798 bp DNA fragment was amplified in each of genomic DNA extracted from each isolate. There was no difference between aflatoxigenic and nonaflatoxigenic species in RAPD test. The marker could differentiate between A. flavus and A. parasiticus.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | PCR, RAPD marker, HPLC, aflatoxin, Aspergillus spp. Pistachio nuts |
| Subjects: | Q Science > QK Botany |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Oct 2016 08:40 |
| Last Modified: | 25 Oct 2016 08:40 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/906 |
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