Shahkarami, F. (2014) Molecular detection of ica operon genes associated with biofilm formation of Staphylococcus Spp. isolated from clinical samples in Zabol city. Masters thesis, University of Zabol.
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Abstract
Staphylococcus spp are recognized as the most frequent causes of biofilm associated infections. This exceptional status among biofilm-associated pathogens is due to the fact that staphylococci are frequent commensal bacteria on the human skin and mucous surfaces. The ability to form a biofilm seems to play an essential role in staphylococcal infections Polysaccharide Intercellular Adhesion (PIA), which is an important component of the staphylococcal biofilm, which is synthesized by enzymes encoded by the ica operon. Biofilm related infections are reversible and usually difficult to respond to the treatment. In this study, the biofilm-forming capacity of 100 clinical isolates of Staphylococcus spp was examined. All Staphylococcus aureus (27 isolates) and Staphylococcus epidermidis (73 isolates) were identified by traditional microbiological methods. Staphylococcal isolates were tested for their ability to produce biofilm using the Congo red (CRA) and Test tube (TM) assay methods. The presence of icaA, icaD, icaB, icaC and icaR genes was determined by PCR technique. One hundred staphylococcal (27 S. aureus and 73 S. epidermidis) isolates, were identified from 100 clinical samples. Out of 100 staphylococcal isolates, 68٪ were biofilm producers. A total 16%, 19%, 14%, 12%, and 11% were respectively positive for icaA, icaD, icaB, icaC and icaR. By PCR method, we identified that among 68% biofilm producer isolates (TM and CRA) 27% and 30% were positive for ica operon and 45% TM+ 65% CRA+ also were negative for ica operon. The results obtained showed that inconsistent with other researches; biofilm formation in Staphylococcal isolates was not associated with present of ica operon and presumably depend on several factors.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Biofilm formation, Ica operon, CRA, TM, Staphylococcus |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 24 Oct 2016 06:59 |
| Last Modified: | 24 Oct 2016 06:59 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/877 |
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