Saboori Robat, E. (2012) Genetic variation assessment of durum Wheat cultivars for Glutenin genes using STS markers. Masters thesis, University of Zabol.
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Abstract
In the present study, the allelic variation within high-molecular-weight Glutenins (HMW-Gs) was investigated in 30 durum wheat (Triticum turgidum var durum) wild type genotypes using STS-PCR. Four pairs of primers from GLU-A1 and GLUB1 genes were exploited for PCR. The results witnessed a remarkable variation among the studied durum wheat genotypes. The variation was analyzed using multivariate methods. Principal component analysis resulted in two principal components, explaining 66.9% of the variations collectively; where 41.38% and 25.52% of the variation was corresponded to first and second components, respectively. Cluster analysis was performed using UPGMA method. In 0.62 similarity coefficient (Jaccard's method) the genotypes were subdivided into eight subgroups. GLU-B1 locus expressed a considerable variation. The genotyped variation was partially correlated with geographical distribution.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | STS, Molecular marker, genetic distance, Protein, Glutenin |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 12 Jun 2016 05:27 |
| Last Modified: | 12 Jun 2016 05:27 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/749 |
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