Heidary, M. (2012) Expression and polymorphism analysis of IL-1B gene and immortalization of B lymphocytes via EBV virus in Sistan and Baluchistan Multiple Sclerosis patients. Masters thesis, University of Zabol.
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Abstract
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system with unknown etiology. At the present time, MS is considered as a multi- factorial disease in which genetic and environmental interactions play an important role in the autoimmune mechanisms of the disease. The autoimmune nature of multiple sclerosis introduces cytokine genes as logical candidates for the loci determining susceptibility to the disease. The role of cytokines gene polymorphisms in MS has been previously reported in the various populations. In this study, we analyzed the association of rs16944 polymorphism in promoter region of IL-1B in 114 patients and 127 controls from Sistan and Baluchistan population and also the expression levels of IL- 1B in 30 MS patients and 30 age and sex matched controls. The rs16944 Polymorphism of IL-1B is genotyped by PCR-RFLP method using AvaI Enzyme and the expression of IL-1B were quantified by RT-PCR method in peripheral blood mononuclear cells (PBMCs). Our results revealed that there is no association between rs16944 polymorphism and MS susceptibility but the expression level of IL-1B were increased in MS patients compared with healthy controls.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Multiple Sclerosis, polymorphism, gene expression, IL-1B |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 12 Jun 2016 04:51 |
| Last Modified: | 12 Jun 2016 04:51 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/742 |
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