Khooshe Bast, Z. (2015) Evaluation of Beauveria Bassiana pathogenicity and toxic effect Zinc Oxide nanoparticles against greenhouse whitefly Trialeurodes Vaporariorum (Hom: Aleyrodidae). Masters thesis, University of Zabol.
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Abstract
The greenhouse whitefly, Trialeurodes Vaporariorum, is a major pest of horticultural and ornamental plants frequently found in fields and greenhouses and usually controlled with pesticides or biological control agents. The current study examined the individual effects of the synthesized zinc oxide nanoparticles (ZnO NPs) and pathogenicity of Beauveria Bassiana TS11 on T. Vaporariorum adults. ZnO NPs were synthesized by precipitation method and were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) and field emission scanning electron microscope (FESEM). The FESEM images indicated that ZnO NPs were non-compacted uniformly. XRD results confirmed the hexagonal wurtzite structure of ZnO NPs with an average size of 23.34 nm. FTIR analysis showed an intense absorption peak at a range of 434-555 cm-1 which was related to Zn-O bond. In bioassays, the adults greenhouse whiteflies were exposed to various concentrations (3, 5, 10, 15, and 20 mg/L) of synthesized ZnO NPs and concentrations (104, 105, 106, 107, and 108 spores/ml) of B. Bassiana TS11 using spray suspension. Based on the obtained results, LC50 values for synthesized ZnO NPs and B. Bassiana TS11 were 7.35 mg/L and 3.28×105 spores/ml, respectively. In addition, LC25 values of ZnO NPs and fungus isolate were 3.76 mg/L and 0.106×105 spores/ml, respectively. Mortality rates of ZnO NPs and B. Bassiana TS11 at the highest concentration were obtained to be 91.6% and 88.8%, respectively. The results indicate the positive effect of synthesized ZnO NPs and B. Bassiana TS11 on T. Vaporariorum adults there by having the potential to be an effective control agent. The current study was conducted under laboratory conditions, therefore, after further field experiments, these control agents can be employed in integrated pest management programs.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Entomo-pathogenic fungus, Nanoparticle, Metal oxide, Insecticide, Bioassay |
| Subjects: | Q Science > QL Zoology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 28 May 2016 08:14 |
| Last Modified: | 28 May 2016 08:14 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/684 |
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