Sadeghi, F. (2011) Effect of natural antifreeze plant treatment on CBF1 gene expression level in different tomato cultivars using Real Time PCR. Masters thesis, University of Zabol.
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Abstract
Low temperature is an important environmental barrier for plants survival, and limitation in their production. Plants adapted to low temperate by activation of a mechanism that will result in increasing tolerance to frost. This adaptation is the expression of some genes that are responsible for taking reaction against stress. In this study, the expression patterns of CBF1 gene in 16 selected tomato genotypes were investigated, cold stress and treated with Thiofer, a natural plant antifreeze with control, were used as treatments, and the expression of CBF1 gene was done using Real Time PCR. In all samples RNA gene was used as the reference gene for the normalization of gene expression. The experiment was factorial design based on randomized block design complete with three replications. The ANOVA was performed using SAS software and cluster analysis was performed by NTSYS software and treatment comparison test was done by Duncan multiple range tests at 5% level. These results suggest that cold stress and natural antifreeze has a positive effect on the expression of genes involved in the CBF1 in response to cold stress. Among the studied genotypes, the highest level of gene expression, in the two treatments, was related to the cold stress and natural plan antifreeze. In genotypes 950, it was 4.2 and 5.7, respectively; it was higher than the control. The lowest CBF1 gene expression in the two treatments, cold stress and natural antifreeze, in genotype Cheffalat, was 1.07 and 2.01, respectively, higher than the control. The results suggest that the antifreeze treatment improves frost tolerance disregarding of the genotype.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | CBF1, cold stress, natural antifreeze plant, tomato, Real Time PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 14 May 2016 09:15 |
| Last Modified: | 14 May 2016 09:15 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/588 |
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