Moshtaghi, F. (2015) Effect of Nigella sativa hydro alcoholic extract on caspase3 gene expression in MCF7 breast cancer cell line. Masters thesis, University of Zabol.
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Effect of Nigella sativa hydro alcoholic extract on caspase3.pdf Download (190kB) | Preview |
Abstract
Breast cancer is the most common cancer, causing deaths among women and men each year. Breast cancer treatments have side effects and drug resistance, therefore finding more effective drugs with fewer side effects is necessary to extend the life of these patients. So the researchers are attracting to use medicinal plants without side effects. One of the mechanisms of anticancer drugs action is killing cancer cells by inducing apoptosis. Studies have shown that the Nigella sativa has cytotoxic and apoptotic properties, because of the compounds such as thymo-quinone and linoleic acid. Caspase-3 genes, is one of the genes involved in apoptosis pathway. The purpose of this study was to evaluate the cytotoxic effects of Nigella sativa extract on the viability and caspase-3 gene expression changes in MCF7 breast cancer cells. Therefore MCF7 cells were treated with different concentrations of ethanol extracts of Nigella sativa (0, 200, 400 µg/mL) for 24, 48 and 72 h. Effect of extract on cell viability and caspase-3 gene expression were analyzed by trepan blue staining and Real time PCR, respectively. The viability results showed that the Nigella sativa extract treated cells in comparison to control cells showed morphological changes of cell death. Nigella sativa extract at concentrations of 200 and 400 µg/mL significantly reduced the viability of cancer cells at different times. The results of Real time PCR showed that the expression of caspase-3 under different concentrations of extract (200 and 400 µg/ml) was significantly increased compared to the control group. Based on the results, Nigella sativa can be used for treatment of breast cancer.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | breast cancer, Nigella sativa, Real time PCR, caspase-3 |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 24 Apr 2016 05:39 |
| Last Modified: | 24 Apr 2016 05:39 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/493 |
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