Boraei-Nezhad, Golnaz (2022) Prevalence of Salmonella infection in traditionally maintained chickens in the Sistan region and determination of the tetracycline resistance genes in the Salmonella isolates. Doctoral thesis, university of zabol.
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Prevalence of Salmonella infection in traditionally maintained chickens in the Sistan region and determination of the tetracycline resistance genes in the Salmonella isolates.pdf Download (445kB) | Preview |
Abstract
Recently multi drug resistant Salmonella species have been emerged due to overuse of antibiotics in veterinary applications which has adverse consequences on human health. The present study was conducted with the aim of investigating the prevalence of Salmonella infection in traditional poultry farms in Sistan region and determining the antibiotic resistance in salmonella isolated from these poultry farms. In this study, 100 local chickens were randomly selected from five counties of Sistan region. A swab sample was taken from each bird by rotating the swab several times in the cloaca. In addition, in each case, some information such as: county, age, gender, breed, proximity to other birds, proximity to waterfowl, proximity to livestock, receipt of antibiotics, especially tetracycline, in the past month were obtained by a questionnaire. The samples were placed in a container in vicinity the ice and transported to the laboratory and cultured using by selective culture media for Salmonella.Then, DNA extraction and PCR were performed on the samples. Out of the total of 100 samples examined, 27 samples were confirmed to be infected with Salmonella by both culture and PCR methods. The results of the present study showed that proximity to waterfowl (OR = 0.273) significantly reduces the risk of salmonella infection. It was also found that the percentage of tet-A and tet-B genes in semi susceptible and resistant isolates is high compared to the sensitive isolates, but this difference is not statistically significant.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | Salmonella, broilers, Tetracycline resistant, Sistan |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 11 Sep 2023 07:22 |
| Last Modified: | 11 Sep 2023 07:22 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3343 |
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