Study of cytotoxic effect of polymer nano capsules and Paclitaxel on malignant human ovarian carcinoma cell (SKOV-3)

Golshan Ara, M. (2021) Study of cytotoxic effect of polymer nano capsules and Paclitaxel on malignant human ovarian carcinoma cell (SKOV-3). Masters thesis, University of Zabol.

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Abstract

Scientist applied various methods for cancer treatment, apoptosis induction or immune system cancer cells eliminating is the backbone of the methods. Mainly, the reason of drug resistance, immune system suspending and therapy failure is heterogeneity, genomic instability in cancer cells which responsible for apoptosis signal suspending and low drug efficiency. Nanotechnology by applied Nano-carrier , developed the drug delivery in medicine, the benefit of that used for cancer treatment methods such as chemotherapy, gene therapy, and immunotherapy. Paclitaxel and other anti-cancer agent must be targeted cancer cells only, because complications of anti-cancer drug impact on all of normal cells in body. In this study we investigated the cytotoxicity of poloxamer F127 Nano-capsule & paclitaxel on SKOV-3 cells line. Cytotoxicity effect determined by MTT assay, we also used caspase activity and qPCR for three oncogene contain MECOM, PRKCl , MYC to find out more about apoptosis signals, that impact by nano-capsule & paclitaxel. Moreover by applied Fluorescence microscopy observed apoptosis induction in cells. Nano-capsule is three black poloxamer that was made in university of Zabol. Our finding indicated 6 pathway for Nano-capsule & paclitaxel on SKOV-3 cells line included: 1.dependent caspase pathways contain 3 & 9 caspases 2.Ras pathway 3.Repressive of P3Ik pathway 4.extronic apoptosis pathway related to caspase 8 5.MAPK pathway 6.prevent tubulin depolymerization and interfering in TGF-β pathway.

Item Type: Thesis (Masters)
Uncontrolled Keywords: MYC, PRKCl , MECOM ,MTT assay , qPCR , Caspases 3,8,9 activity, Light microscopy , Fluorescence microscopy
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Mrs najmeh khajeh
Date Deposited: 02 Oct 2022 06:48
Last Modified: 02 Oct 2022 06:48
URI: http://eprints.uoz.ac.ir/id/eprint/3110

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