Kheirgoo, Masumeh (2021) Association mapping of the genes controlling resistance to leaf and spike diseases in wheat. Doctoral thesis, University of Zabol.
|
Text
Association mapping of the genes controlling resistance to leaf and spike diseases in wheat.pdf Download (136kB) | Preview |
Abstract
Septoria tritici Blotch and Fusarium Head Blight are important and damaging diseases of wheat in all major wheat growing areas including Iran. The most appropriate method for controlling both of disease, bearing in mind bioenvironmental and economic consideration, is using of wheat resistant cultivars. This study was conducted to identify and association mapping resistance genes to these diseases. The genotypes were cultivated in an augment design in the research farm of Aliabad Katoul Agricultural School. Artificial contamination of the genotypes were done using spreading infected leaves and inoculating with a pathogenic fungal spore suspension. Response of the genotypes to disease-causing were noted. The results of analysis of variance and cluster showed that the genotypes were very different in terms of traits related to septoria trittici disease and there was a great genetic diversity in terms of resistance to this disease among the studied genotypes. Comparison of means using Duncan's test showed that group 3 was resistant to septoria. Genotypes 51, 68, 79, 85, 87, 89, 97, 110, 115, 116, 123 and 130 were included in this group and were identified as sources of resistance to leaf septoria. Analysis of variance and cluster also showed that there was a great genetic diversity among genotypes in terms of Fusarium disease resistance.Comparison of means using Duncan's test showed that group 3 was resistant to fusarium. Genotypes No. 3 (Hirmand), 5 (Maroon), 6, 7 (Arta), 11 (Gohar), 12, 13 (Golestan), 33, 39, 40 (Qaboos), 44 (Pishtaz), 49, 52 (Shush) 59, (Pasteur), 88 (Karaj 2), 91, 92, 99, 127 and control C (Ehsan) were included in this group and were identified as sources of resistance to Fusarium. Eighty studies that identified Fusarium-associated QTLs were studied. Of these, 53 studies (66%) had the information needed to QTL projection. The results of meta-analysis showed that the linkage groups 3, 2 and 5 with 33, 25 and 20 QTLs respectively had the highest number of QTLs. The number of 4, 4 and 2 meta QTLs were identified on 2D, 3B and 5A chromosomes, respectively. MQTL6 was located on chromosome 3B and explained The highest phenotypic variance of the trait.A marker-trait association was detected for sAUDPC, rAUDPC and AUDPC in septoria. The Xbarc263 marker showed a significant relationship with these traits. This marker was located on the consensus map used for the MQTL analysis at position 27 cm on chromosome 1A.The markers Xbarc173-6D, Xgwm512 and 47-210-3 showed a significant relationship with the trait of days to heading. The markers Xbarc173-6D and Xgwm512 were located on chromosomes 6D and 2A, respectively. Xbarc173-6D and 47-210-3 markers, in addition to days to heading traits, also showed a significant relationship with AUDPC, sAUDPC and rAUDPC traits of Fusarium disease.The markers Xgwm5-3A, Xbarc171 and Xbarc173-6D, which were located on chromosomes 3A, 6A and 6D, respectively, showed a significant relationship with the severity of the disease in Fusarium disease. Disease index trait showed a significant relationship with Xbarc171 and Xbarc108 markers. These markers were located on chromosomes 6A and 7A, respectively. The markers Xgwm190, Xbarc173-6D and 47-210-3 showed a significant relationship with AUDPC, sAUDPC and rAUDPC traits of fusarium disease.
| Item Type: | Thesis (Doctoral) |
|---|---|
| Uncontrolled Keywords: | Zymoseptoria tritici Blotch, Fusarium Head Blight, Molecular markers, Population structure |
| Subjects: | Q Science > QK Botany |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 19 Sep 2022 06:14 |
| Last Modified: | 19 Sep 2022 06:14 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2984 |
Actions (login required)
 |
View Item |
