Insecticidal and repellency effects of Lonicera caprifolicum and Rhazya stricta extracts on Tribolium castaneum and Sitophylos oryza

Roshanbar, M. (2013) Insecticidal and repellency effects of Lonicera caprifolicum and Rhazya stricta extracts on Tribolium castaneum and Sitophylos oryza. Masters thesis, University of Zabol.

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Insecticidal and repellency effects of Lonicera caprifolicum and Rhazya stricta extracts on Tribolium castaneum and Sitophylos oryza.pdf

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Abstract

Application of herbal compounds could be one of the best methods for environment’s protection from pesticide injuries. To assess the effect of herbal compounds as natural insecticide and repellents on red flour weevil Tribolium castaneum and rice weevil Sitophylus oryzae leaf extract of Lonicera caprifolicum and Rhazya stricta were studied under 27±1° C and the humidity 65±5%. Herbal extraction was done using ethanol and acetone solvents. Contact toxicity tests were done on 1 to 7 days old of adult insects. The results showed a significant and positive relation between concentration of herbal extracts and mortality of adults after 24h of application. The LC50 values of contact toxicity tests with ethanol and acetone solvents of R.stricta extract on T.castaneum and S.oryzae were found to be 147.87, 75.90 and 136.88, 82.19 mg/cm2, respectively. Furthermore the LC50 values of the contact toxicity tests with ethanol and acetone solvents of L.caprifolicum on T.castaneum and S.oryzae were demonstrated 349.78, 108.05 and 106.2, 34 μg/cm2, respectively. Leaf extract of L.caprifolicum with acetone had highest repellency percentage on T.castaneum and S.oryzae. GC-Mass technique was used for identification of the components of the extract. The most abundant compounds of R.stricta extract were detected as Eburnamenin, Ibogamine, Quebrachamine, 17-Methoxyquebrachamin, Hydroxylamin, Aminoxybutiric acid, Phenol, (2-Ethylhexyl) phthalate, Dimethlpropyl with a percentage of 62.56, 0.47, 9.25, 3.17, 1.08, 1.67, 4.17, 1.47 and 1.72, respectively. In L.caprifolicum extract, trans-Nerolidol, Linalool, p-Cymene, Eugenol, Hexadecanoic acid, Geraniol, Acetophenone, Cis-Jasmone and Globulol were found with maximum percentage of amount 17.1, 9.8, 7.5, 6.6, 6.4, 5.3, 3.2, 2.4 and 2.9, respectively. Our results suggested that herbal extract have high repellency and toxicity effects on T.castaneum and S.oryzae. Therefore, it is recommended to use such extract compounds for application on the pests.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Insecticide, repellency, herbal extract, Tribolium castaneum, Sitophylus oryzae, bioassay, Lonicera caprifolicum, Rhazya stricta
Subjects: R Medicine > RB Pathology
Depositing User: Mrs najmeh khajeh
Date Deposited: 13 Sep 2022 06:37
Last Modified: 13 Sep 2022 06:37
URI: http://eprints.uoz.ac.ir/id/eprint/2942

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