Mesbah, Najmeh (2020) Evaluation of Enterobacteriaceae contamination in carrot juice supplied in Zabol juice stores and their antibiotic resistance. Masters thesis, University of Zabol.
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Abstract
In this study, we investigated the rate of Enterobacteriaceae infection and their antibiotic resistance in carrot juice offered in juice stores in Zabol city. We obtained 100 samples of carrot juice in juice stores in Zabol city them to the Health and Quality Control Laboratory of the Faculty of Veterinary Medicine, University of Zabol. The samples were weighed at 25 g and then added to 225 ml of lactose broth or peptone water. It was then added to EMB, Selenite Cystine Broth and Tetrathionate broth culture medium to isolate Escherichia coli and to search for Salmonella bacteria. We then added it to SS agar and bismuth sulfite agar and brilliant green agar media and cultured linearly. Also, for Escherichia coli confirmation tests, colonies with metallic green polish were transferred to Simon Citrate, TSI, SIM and finally to MR-VP culture medium. For Salmonella confirmation tests, colonies grown in SS agar, brilliant green agar, and bismuth sulfite agar were deep and shallow cultured on TSI and LIA culture media. Finally, the susceptibility of the obtained isolates to the following antibiotics was assessed: nalidixic acid (NA), ampicillin (AM), Amikacin (AN), Imipenem (IMP), Ciprofloxacin (CP), Gentamicin (GM), Ceftriaxone (CRO), Tetracycline (TE), Amoxiclav (AMC), and Sulfamethoxazole (SXT). The findings of this study showed that out of 100 samples of carrot juice studied in this study, 72 cases (72% with 95% confidence interval from 63% to 80%) were infected with Salmonella and 5 cases (25% with 95% confidence interval from 1% to 9%) were infected with Escherichia coli. Salmonella was most resistance to AM, TE and AN ,SXT isolates For Escherichia the most allergy was related to antibiotic.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Carrot juice, Salmonella, Escherichia coli, Bacterial resistance |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 19 Oct 2021 07:32 |
| Last Modified: | 19 Oct 2021 07:32 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2794 |
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