Harati, Mojtaba (2019) Prevalence of virulence genes fimbriae in Escherichia coli strains isolated from poultry meat in sistan. Masters thesis, university of zabol.
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Abstract
The first stage of the infection with Escherichia coli is the binding phase. For this, Fimbria Factor plays a major role in the bacterial binding. In this study, the prevalence of Fimbria virulence genes of Escherichia coli isolates isolated from Poultry meat in Sistan province was investigated. From 1,500 , 100 isolates of Escherichia coli were studied. After the biochemical examination of the isolated bacteria, DNA extraction was performed from isolates. Subsequently, polymerase chain reaction was performed for fim, pap, foc, afa and sfa genes. The results showed that fim gene with 100% frequency had the highest statistical percentage And then the Pap gene is the most frequent statistically. In the present study, the prevalence of Escherichia coli fim gene in Poultry meat in the Sistan region was high. After that, the prevalence of the virulence gene of Pap Escherichia coli in Poultry meat in the high Sistan region, which accounts for 22% of the frequency of this gene, and the remaining virulence genes Zero percentages were observed. In the PCR reaction, three foc, afa and sfa genes did not show any bands and were negative. In the present study, the reaction was based on the distribution of virulence genes from 100 isolates of Escherichia coli isolated from Poultry meat referred to 2 Different genetic distribution patterns were observed. This study showed that the prevalence of Viverulence fimbriae genes of Escherichia coli isolated from Poultry meat in Sistan region for fim and Pap genes.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Escherichia coli, Virulence, Polymerase Chain Reaction, patterns of genes Poultry meat |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 31 Dec 2019 05:47 |
| Last Modified: | 31 Dec 2019 05:47 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2665 |
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