saleki, Rasoul (2019) Prevalence of Virulence Genes in Avian pathogenic Escherichia coli (APEC) Strains Isolated from broiler chickens around Zabol. Masters thesis, university of zabol.
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Molecular identification and evaluation of antioxidant and antimicrobial.pdf Download (170kB) | Preview |
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Prevalence of Virulence Genes in Avian pathogenic Escherichia coli.pdf Download (243kB) | Preview |
Abstract
Escherichia coli bacteria are as a normal flora in intestine of mammals and birds, and most strains are not pathogenic while some strains can cause different disease such assepticemia, peritonitis, salpingitis, synovitis, omphalitis, coligranuloma and yolk sac infection.The main goal of this study was to determine the abundance of virulence factor encoding genes including, toxins (cnf1) and hemolysins (hlyA), iron uptake system (iucD, iroN and irp2), adhesins (fimH and iha) and outer membrane protease (ompT) in Escherichia coli isolated from broiler flocks around Zabol. A total of 100 Escherichia coli isolates were obtained from broiler flocks and boiled for DNA extraction and then all genes were evaluated by Multiplex PCR. The results showed that among the 100 samples, fimH was the most abundant gene with 94% and the iha gene was the least abundant with 21%. The two cnf1 and hlyA genes were not found in any of the isolates. Frequency of other genes were also observed for iroN, irp2, ompT, iucD genes with frequency of 74, 57, 48 and 42%, respectively. Since most of these genes have not been comprehensively studied in the region so far, the results of this study can be used to adopt preventive and therapeutic strategies and improve the poultry production efficiency and consequently the use of appropriate medicines widely used to control the disease in the area.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Virulence factor, Avian pathogenic Escherichia coli (APEC), broiler chickens, Zabol |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 16 Nov 2019 06:10 |
| Last Modified: | 16 Nov 2019 06:10 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2581 |
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