Nikbakht, Behzad (2019) Investigating the rate of pouring of beef produced in Zahedan to Escherichia Coli using MPN and detection of O157: H7 by PCR. Masters thesis, University of Zabol.
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Abstract
Beef contamination usually occurs in the killing process, one of the intestinal bacteria that cause it to spread in humans and animals everywhere, is an E. coli bacterium. One of the important serotypes of this bacterium is Ecoli O157:H7. The aim of this study was to determine the rate of contamination of beef produced in Zahedan to Escherichia coli by MPN Methode and also isolated of O157:H7strain by PCR. During this study, from July 1396 to July 1397 Within six months, 360 beef samples from red meat packages of Zahedan city, from different parts of the carcasses The isolation of the heat-resistant Escherichia coli in the laboratory was carried out using the MPN method. Seventy isolates were finally isolated. The final test was performed using a specific primer for rfb to detect Ecoli O157:H7 using a PCR reaction. Only one isolate from The opinion of this serotype was confirmed, which indicates the proper health status of the meat produced in Zahedan
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Escherichia coli, O157: H7, MPN, PCR Method, Zahedan |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 06 May 2019 07:13 |
| Last Modified: | 06 May 2019 07:13 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2426 |
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