Drug resistance and prevalence of blaSHV and blaOXA gens of Escherichia coli isolated from broilers

Noori, Hossein (2018) Drug resistance and prevalence of blaSHV and blaOXA gens of Escherichia coli isolated from broilers. Doctoral thesis, university of zabol.

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Abstract

Escherichia coli is a natural flora of the alimentary tract of birds and is one of the most common pathogens in poultry that causes Colibasilosis. It is also a bacterial pathogen in humans, especially in immunodeficiency patients. Extendedspectrum beta-lactamases (ESBLs) are widely used to treat infections caused by this microorganism. Most ESBLs can be divided into three groups of SHV, TEM, and CTX-M, based on the similarity of the amino acid sequence, respectively, coded by blaSHV, blaTEM and blaCTX-M genes. Regarding the development of antibiotics resistance and harmful effects of resistant bacteria on beta-lactam antibiotics, they can have an effect on human health and animal health, and due to the high mortality rate of disease, Reducing production, as well as damage caused by drug use, it’s very important to control and prevention this disease. In the present study, 50 isolates of Escherichia coli were collected from 60 cloacal swabs from broiler chickens between 7 to 21 days old in Zabol city. Antibiogram test was performed by disk diffusion method to investigate drug resistance to Ceftriaxon, Cefazolin, Ceftizoxime, Cephalothin, Ceftazidime and Cefixime, which was the highest resistance to Cefazolin and the least resistance to Ceftriaxon. The frequency of two blaSHV and blaOXA genes was evaluated by PCR method. The results showed that the frequency of blaSHV and blaOXA gene in the isolates was 24% for blaSHV and 0% for blaOXA.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: Escherichia coli, Extended-spectrum beta-lactamase(ESBLs), blaSHV, blaOXA, PCR
Subjects: S Agriculture > SF Animal culture
Depositing User: Mrs najmeh khajeh
Date Deposited: 15 Oct 2018 05:44
Last Modified: 10 Mar 2019 07:28
URI: http://eprints.uoz.ac.ir/id/eprint/2191

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