Enayat avval, S. (2015) Study of genetic diversity in landraces of Citrullus colocynthis using SSR molecular marker. Masters thesis, university of zabol.
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Study of genetic diversity in landraces of Citrullus colocynthis using SSR molecular marker.pdf Download (140kB) | Preview |
Abstract
Citrullus colocynthis, is a herbaceous plant of the Cucurbitaceae family, and the primary source of Citrullus colocynthis is archipelago located in the southern part of Bhutan. As a food vegetable, and for its medicinal properties, including a therapeutic effect in patients with diabetes, and cholesterol-lowering effect in patients with hypercholesterolemia, Citrullus colocynthis is important, and is cultivated in tropical and subtropical areas. In this study, to study the genetic diversity of Citrullus colocynthis Native Masses, Simple sequence repeat marker or (SSR) was used. The SSR is the most widely used Marker that is very diverse, distributed in eukaryotic genomes, and is dedicated to the locus. In this study, the population studied, Citrullus colocynthis was collected randomly from native masses of 8 regions in Sistan and Baluchestan. Extracting DNA from samples was performed by using as cetyl trimethyl ammonium bromide method (CTAB). A total of 10 primers were designed and used in this study. Polymerase chain reaction was studied using DNA extracted and 10 primers. For the analysis of genetic data, NTSYS pc ver 2/2 and Genalex 6.5 and XLSTAT software was used. According to the data analysis among cultivars studied, and because of that, 7 primer of 10 primers used in the study showed polymorphism among the studied samples, it was not possible to classify them. Thus, results of the present study confirm the great genetic variation in under study samples of Citrullus colocynthis plant. It seems that this genetic diversity is probably unique to Sistan and Baluchestan Province and in general toour country and Iran plateau.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Citrullus colocynthis, genetic diversity, SSR molecular marker |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 28 May 2017 08:08 |
| Last Modified: | 28 May 2017 08:08 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1385 |
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