Ahmand, Z. (2016) Genetic diversity of different populations of Cuminum cyminum and Carum carvi using microsatellite markers. Masters thesis, University of Zabol.
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Abstract
to evaluate genetic diversity amang plant populations is many different methods that Molecular marker were used to investigate of genetic diversity and similarity amang differentiat species. Microsatellite markers are based on PCR which identify areas with high diversity. The abjective of this research was to investigate the genetic variation at molecular level using SSR marker among 10 genotypes of Cumin (five of which were cuminum cyminum and the vest were Bunium persicum) collected from different region of Iran. DNA was extracted from leaf of 20-day siddling based on Dellaporta methods. To evaluation of quality and quantity of DNA was used agarose gell and spectrophotometry and after that was used 12 SSR markers for DNA analysis of all genotype. All 12 SSR markers generated 59 bands that were different from 3 to 8 band per perimer with average 4.9 bands. The average of diversity index and Genetic similarity among Cuminum cyminum was respectively 0.61 and 0.292 and also was 0.61 and 0.311 respectively for Bunium persicum. According to cluster analysis, all genotype were clustered into three groups and the results of PCA showed that 65% of total variance were conducted by three of primery components analysis. Results of this study indicated that, based on microsatellite marker wrer not significant different between cuminum cyminum and Bunium persicum.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Cumin, SSR marker, Microsatellite, Diversity |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 17 Apr 2017 05:23 |
| Last Modified: | 17 Apr 2017 05:23 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1235 |
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