Effect of nisin and alcoholic extract of hibiscus sabdariffa on inoculated populations of staphylococcus aureus ATCC 1113 in litopenaeus vannamei minced fillet at 4°C.

Sargazi, S. (2016) Effect of nisin and alcoholic extract of hibiscus sabdariffa on inoculated populations of staphylococcus aureus ATCC 1113 in litopenaeus vannamei minced fillet at 4°C. Doctoral thesis, University of Zabol.

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Abstract

Staphylococcus aureus is an important born pathogen for food industry and is a member of hazand group food born bacteria. This study aimed to the antibacterial activity of alcoholic extract of hibiscus (hibiscus sabdariffa) in inhibiting of S. aureus (Staphylococcus aureus) alone and in combination with nisin in minced meat vannamei shrimp was done. Extraction of Extracts of plants was performed with using vacuum evaporation was conducted with the help of Rotary effect of different concentrations of alcoholic extracts of hibiscus (0, 1, 5/2, 5/3, 5%) and solution of nisin (0, 5/0, 5/2 µg/g) and combining varying amounts of solution nisin (µg/g) and extracts (%): (0/25+3/5), (0/25+5), (0/5+1), (0/5+2/5), (2/5+1), (2/5+2/5) on grow thing behavior of the Staphylococcus aureus at 4° c temperature over 21days was investigated. The results showed that bacterial growth at extracts of hibiscus (3/5, 5) and solution of nisin (0/5, 2/5) after six day has stopped on Low concentrations of extracts of hibiscus (1and 2/5%) did not have significant differences to inhibit the bacterial growth. Combined treatment were effective after six days to stop the growth of Staphylococcus aureus. According to the findings of this research, hibiscus extract and solution of nisin lonely has an inhibitory properties and those have natural preservative effects on food instead of industrial chemicals additives. This research approved the synergistic effect of nisin and alcoholic extract on the inhibition of the bacterial growth.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: litopenaeus vannamei, Staphylococcus aureus, Hibiscus sabdariffam, Nisin, alcoholic Extract
Subjects: S Agriculture > S Agriculture (General)
Depositing User: admin admin1 admin2
Date Deposited: 29 Nov 2016 05:53
Last Modified: 29 Nov 2016 05:53
URI: http://eprints.uoz.ac.ir/id/eprint/1038

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