Rahdari, Abdolali (2011) Investigation on artificial breeding of the Sistan snow trout (Schizothorax zarudnyi) using synthetic hormones. Masters thesis, University of Zabol.
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Abstract
The Snow trout (Cfidak-e-Sistan) (Schizothorax zarudnyi Nikolskii, 1897) is an endangered species of fish endemic to Hamoon Lake, Sistan, Iran. Natural fish populations have declined during the last several years because of the environmental degradation due to drought and introduction of non-endemic fishes to Hamoon Lake. The purpose of this study was to evaluate the use of synthetic hormones, ova-prim and human chorionic gonadotropin (hCG) to induce spawning and determination of some reproductive parameters. Wild broods of snow trout (females and males 1328±45, 632±17.6 g respectively) were caught in the Chahnimeh reservoirs (Sistan) and transported to Zahak hatchery for artificial spawning. The Brood fish were divided into five groups and treated with: a) Quadruplet injections of different doses 0.2, 0.5, 0.5 and 0.3 ovaprim/kg fish body weight, 24 h intervals for females. Males injected 0.3 ml/kg ovaprim simultaneous second females' injection. b) Triplet injections of ovaprim and hCG with different doses; 0.2, 0.5 and 0.5 ml/kg ovaprim+1000, 2000 and 2000 I.U./kg of females body weight, 24 h intervals for females. Males injected 0.3 ml/kg ovaprim + 1500 I.U. hCG/kg of body weight simultaneous second females injection's) Quadruplet injections of ovaprim and hCG with different doses; 0.2, 0.5, 0.5 and 0.3 ml/kg ovaprim+200, 400, 400 and 300 I.U. hCG/kg of females body weight, 24 h intervals for females. Males injected 0.3 ml/kg ovaprim + 200 I.U.hCG/kg of body weight simultaneous second females injection. d) Triplet injections of hCG with different doses 400, 800 and 800 I.U./kg of females body weight, 24 h intervals for females. Males injected 500 I.U./kg of body weight simultaneous second females injection. e), saline injection (control group) 0.3 ml/kg fish body weight. The results showed that the mean working fecundity for a, b, c, d and e groups were 39531±7802, 18625±9704, 15682±5982, 0 and 0, respectively. Latency period were 36.2±3.77, 12.33±0.66 and 30.2±2.95, conversion percentage of dry egg to eyed egg were 88.97±0.9, 73.19±2.04 and 75.26±3.90, conversion percentage of eyed egg to larvae were 81.93±1.15, 75.67±0.19 and 71.03±1.03 for a, b and c groups, respectively. There were significant differences in the fecundity, latency period and other parameters mentioned above between the group treated only with ova-prim and the control group (p<0.05, Tukey´s test, respectively). So treatment of Snow trout with ova-prim is effective for spawning induction.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Schizothorax zarudnyi, Spawning induction, Ova-prim, hCG, Sistan |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Jun 2016 06:56 |
| Last Modified: | 25 Jun 2016 06:56 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/870 |
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