Rezaei Tavabe, Shiva (2023) Lactobacillus plantarum and Lactobacillus fermentum endophyte in zein/polyvinyl alcohol/zein multilayer nanofibers by sandwich method. Masters thesis, university of zabol.
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Lactobacillus plantarum and Lactobacillus fermentum endophyte in zein polyvinyl alcohol zein multilayer nanofibers by sandwich method.pdf Download (856kB) | Preview |
Abstract
Probiotics are live microorganisms when administered in adequate amounts confer a health benefit on the host. Probiotics must be metabolically stable and survive gastrointestinal tract passage in achieving the health-promoting effects on the host. In order to increase the viability of probiotics, the encapsulation of L.plantarum MT.ZH193 and L. fermentum MT.ZH893 in single-layer polyvinyl alcohol (pva) and multilayer )zein/pva/zein( nanofibers was done with an electrospinning machine in the Zabol University. The concentration of zein and pva solutions were 27% and 10% (w/v), respectively. Three voltages of 11, 18, and 25 kV were carried out for electrospinning. Then, the microorganism survival percentage, nanofiber diameter (nm), nanofiber structure by scanning electron microscope, atomic force microscopic (AFM) image analysis, infrared Fourier transform spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffraction, electrical conductivity, and determination of specific surface by the BET method were performed on the samples. The results showed that the survival percentage at voltages of 11, 18 and 25 for single-layer pva nanofibers containing L. plantarum was 0.000±0.00, 35.47±1.33 and 48.78±0.88, and for pva containing L. fermentum was 0.00±0.00, 48.04±1.57 and 55.56±1.38, respectively. Therefore, the highest survival rate was observed at 25 kv voltage (p<0.05). The survival percentage of L. plantarum and L. fermentum in zein/pva/zein multilayer nanofibers at 25 kV voltage was obtained, 73.78±1.05 and 85.64±1.22, respectively. As the voltage increased from 11 to 25 kV, the diameter of nanofibers decreased (p<0.05). The electron microscope images proved the presence of bacteria in nanofibers. The atomic force microscopic (AFM) images showed that nanofibers containing bacteria had less surface roughness than the control. The Fourier transform infrared spectroscopy (FTIR) showed that the addition of bacteria into nanofibers had an effect on the secondary structure of pva. The thermogravimetric test showed that zein/pva/zein multi-layered nano fibers containing L. fermentum had higher thermal stability than the control sample. X-ray diffraction showed L. fermentum increased the crystallinity of nanofibers. By adding microorganisms to nanofibers, the electrical conductivity of the samples increased. Samples containing microorganism showed less porosity.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Native probiotics, Electrospinning, Survival percentage, Electrospun sandwich‐structure, Zein, Polyvinyl alcohol |
| Subjects: | T Technology > TP Chemical technology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 29 Sep 2024 16:29 |
| Last Modified: | 29 Sep 2024 16:29 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3638 |
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