Analysis of genetic diversity and structure of ornamental fish by Microsatellite markers

Fazel, S. i. (2014) Analysis of genetic diversity and structure of ornamental fish by Microsatellite markers. Masters thesis, University of Zabol.

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The purpose of this study was to investigate the genetic structure and diversity of ornamental fish is using microsatellite markers. Four species, including rosy, green, tiger and Albino ornamental fish breeding centers in different parts of Mashhad preparation and purification of the 40 subjects were randomly assigned to two generations (10 specimens of each species) were selected and the amount of 0.2 g DNA was extracted from the tissue of the dorsal fin. DNA was extracted from tissue kit company DENA ZIST Asia was friendly. To study the genetic diversity between and within species of four microsatellite markers were used. Amplification of microsatellite sites within the polymerase chain reaction was performed with specific primers and detected four genotypic patterns of PCR products were analyzed on an 8% acrylamide gel. The number of alleles observed at four positions 21 and the total population, respectively, 5, 6, 5 and 5. The number of alleles at position sm17 species Barb and the lowest number of alleles at position Mal06 and in green Barb was kind. Heterozygosis at the sites studied in the range of was 0.16 – 0.3. Polymorphism information content of the four positions in four species are in the four largest SM25 (0.42), Mal06 (0.37), SM17 (0.60) and MA109 (0.46), respectively. All positions except the position of the SM25 and Mal06 in place of Barb Albino and position sm17 population rosy barb of Hardy_ Weinberg equilibrium were deviations. Phylogenetic analysis and AMOVA analysis based on data from four polymorphic loci showed that %3 and 97% of the variation in the diversity within the population, but the population of Green Barb other populations are high genetic similarity percentage.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Genetic diversity،Ornamental fish، Microsatellite
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Depositing User: admin admin1 admin2
Date Deposited: 17 Apr 2016 09:08
Last Modified: 17 Apr 2016 09:08

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