Gazerani, Z. (2014) Pathogenicity interaction of R. solani and Meloidogyne javanica in bean varieties. Masters thesis, University of Zabol.
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Abstract
Common bean (Phaseolus. vulgaris L.) has the most under – cultivation areas among cereals in the world and is very important because of having 22% protein in food diet. Rhizoctonia root rot and crown rot of bean caused by R. solani is one of the most important diseases of this crop in Iran. Root - knot nematode Meloidogyne javanica is the most causal agent of many crops especially bean. Interaction of these microorganisms has been studied in different hosts by investigators in the world. Since the presence of these pathogens in bean seedling were approved in Tehran province. In this study R. solani AG4 isolated were prepared from Tehran University and was cultured on PDA medium. Second stage juveniles (J2) of nematode was used as the source of inoculum was prepared from Shahed University in Tehran and was reared on tomato seedling var. Rutgers. In this study seedling of bean cultivars, Bahman, Daneshkadeh, Dehghan, Naz, Sadaf, Sanray and Sayad, were transplanted to pots containing 1000g of sterilized sandy loam soil. In this experiment a factorial completely randomized design (CRD) with 9 treatments and 3 replication was conducted in a greenhouse. Treatments was as follows: control, nematode alone, fungus alone and nematode+fungus (simultaneously). Pots were inoculated with (0, 1000, 2000) J2 nematode and/or (0, 5, 10 no/g soil) propagate of fungus according to the treatments. Pots were arranged in glasshouse benches with the temperature of 25- 270C and natural light. Experiment was terminated after 2months and following parameters were determined i.e., dry and weight of root and stem, plant height, no galls and egg masses per root system, and necrosis of root tissue and disease incidence by fungus. Results showed that presence of nematode caused reduction on colonization of the fungus in the root and stem and v ice verse i.e., presence of fungus caused reduction on none of galls and egg masses produced the nematode in a treatment. Maximum disease incidence was observed in Dehghan and Sadaf cultivar. Minimum disease incidence was observed in fungus alone treatment of Sayad cultivar. Maximum and of Galling and egg mass in root system were in cvs. Daneshkadeh, Sadaf, respectively.
Item Type: | Thesis (Masters) |
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Uncontrolled Keywords: | Pathogenicity, Rhizoctonia solani, Meloidogyne javanica, bean |
Subjects: | Q Science > QK Botany |
Depositing User: | admin admin1 admin2 |
Date Deposited: | 01 Nov 2016 07:45 |
Last Modified: | 01 Nov 2016 07:45 |
URI: | http://eprints.uoz.ac.ir/id/eprint/955 |
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