Sanchooli, S. (2014) P53 gene expression evoluation in patient with esophageal cancer by reverse transcriptase real - time polymerase chain reaction. Masters thesis, University of Zabol.
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Abstract
Esophageal Cancer with more than 386,000 mortalities around the world is the sixth cause of cancer death. The prevalence of cancer in Iran is so high. In fact, Iran has the highest rate of esophageal cancer in the world. The rate of mutation in P53 has been determined in both kinds of esophageal cancer. P53 as one of the important proteins that is activated against possible damages to DNA is one critical aim for different researches. This kind of cancer like all others is the result of various environmental effects and also genetic changes. In this research, expression of P53 gene as one of the important factors resulting esophageal cancer will be studied in different samples of patient’s paraffinic tissue in Iran. Approximately, 15 samples of paraffinic control and 15 samples of paraffinic tissue collected from various clinical and medical institutes (Zabol, Zahedan and Kashan) to analyze P53expression by Reverse transcriptase real – time polymerase chain reaction method. All PCR reactions have done by three iterations for P53 gene and internal control (B-actin) by (Livac) method. The difference in gene expression in patients and control has been investigated by using T-test. Software SPSS used for all analyses. From statistical point of view there is a meaningful difference in prevalence of expression of P53 gene between patient and control.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Esophageal Cancer, P53 gene, Reverse Transcriptase Real-Time, Polymerase Chain Reaction |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 01 Nov 2016 07:37 |
| Last Modified: | 05 Nov 2016 09:40 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/952 |
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