Identification of Streptomyces spp. of north of Iran and their effects on control of citrus nematode (Tylenchulus semipenetrans)

Bahramyan, H. (2015) Identification of Streptomyces spp. of north of Iran and their effects on control of citrus nematode (Tylenchulus semipenetrans). Masters thesis, University of Zabol.

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Citrus nematode (Tylenchulus semipenetrans) is one of the nematodes that widely spreads in the Citrus gardens of world and Iran. It causes less the yield and decline of trees. The purpose of this study was to investigate the anti-nematode activity against Citrus nematode and identification some local Streptomyces strains isolated from citrus soils. In order to investigate the effect of biological agents on mortality of larvae in summer 1392 from citrus orchards soils in Gilan, Mazandaran and Golestan were sampled from trees with symptoms and without symptoms of nematode. Isolation of Streptomyces in a semi selective medium Casein Glycerin Agar (CGA) was done. 46 Streptomyces strains for studying larval control citrus in vitro were isolated and purified. To study the antagonistic effect test, the citrus Rough lemon seedlings infected with citrus nematode were keep for three months in the greenhouse. The soil samples transferred to laboratory then larvae were isolated from soil by tray method. The test was conducted in a completely randomized design with three replications. 13 isolates in 4 days showed antagonistic ability. Among the 13 isolates, the isolates SP13-2 and SP8-3 as much as 49.66% and isolates SP23-1 with 49.33% mortality of larvae showed the highest antagonistic activity. Identification the effective Streptomyces strains by the Morphological, physiological, biochemical and molecular traits was performed. Then, the region of 16srDNA sequenced and the PCR product of 16srDNA district ribosome of 8 isolates including SP8-3, SP23-1, SP15-1, SP13- 2, SP4-1, SP8-2, SP8-1, and SP16-5 sent to Korea. The results of sequencing referred to the GenBank and after blasting of sequences, these recorded in GenBank (Websites of NCBI).

Item Type: Thesis (Masters)
Uncontrolled Keywords: Citrus nematode, Streptomyces, Biocontrol, Morphologic, Biochemical and molecular
Subjects: Q Science > QK Botany
Depositing User: admin admin1 admin2
Date Deposited: 30 Oct 2016 07:15
Last Modified: 30 Oct 2016 07:15

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