Identification of desert truffle fungi in Golestan province and study of their genetic diversity using molecular marker, SSR

Komaki, A. M. (2010) Identification of desert truffle fungi in Golestan province and study of their genetic diversity using molecular marker, SSR. Masters thesis, University of Zabol.

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Desert truffles are group of hypogenous edible fungi and classified in order Pezizales and included genera Terfezia, Picoa and Tirmania. This group of fungi growing in Mediterranean area from North Africa to Middle East. There are few species of Desert truffle in Iran, unfortunately rare information about them. Therefore, to study of Taxonomy, identification and genetic diversity of Desert truffles collected 119 isolates from Golestan province and 12 isolates from Ilam, Fars and Sistan and Baluchistan provinces from different habitats. The samples was collect from late march until mid-may in 2007-09. In order to morphological study of isolates, get microscopy slides hand section from different part of Fruiting bodies and then studied by using light microscope. To evaluate ascospore germination and produce of colony in different species of desert truffles, a little part of fruiting bodies cultured in Potato- Dextrose-Agar (PDA), Nutrient Agar (NA) and Murashig and Skoog medium´s. In order to study genetic diversity of desert truffles collected from different habitat by using molecular marker short sequence repeat (SSR), 26 isolates in total were selected for molecular studies. Each of isolate were studied with six microsatellite primers such as sequences (CATA)4, (GACA)4, (TGTC)4, (CAG)5, (GAC)5 and (GTG)5. As a result of morphological studies identified three species of desert truffles, species Picoa lefebvrei from Golestan province, species Terfezia claveryi from Golestan, Fars and Ilam provinces and species Tirmania Nivea from Sistan and Baluchistan province. The P. lefebvrei and T. Nivea are new species for Iran´s mycoflor. The ascospores isolated from fresh fruiting bodies of desert truffles T. claveryi and P. lefebvrei germinated and produced colony on artificial medium Murashig and Skoog (MS). The ascospores of desert truffle T. Nivea have not germination. Desert truffles species Terfezia claveryi with (GACA) 4 primer and species Picoa lefebvrei with (CAG) 5 primer had been good polymorphism.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Desert truffle. Taxonomy, Morphology, Mycorrhiza, Pezizales
Subjects: Q Science > QK Botany
Depositing User: admin admin1 admin2
Date Deposited: 26 Oct 2016 04:58
Last Modified: 26 Oct 2016 04:58

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