Meshkinkhood, N. M. (2014) Molecular identification of Saglin and SG1B genes of Anophele Stephensi (vector of malaria) candidates for design transmission blocking vaccines. Masters thesis, University of Zabol.
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Abstract
Malaria is a major problem in today's world. It affects millions of people and causes many deaths in the world each year. Today, due to the evolution of drug resistance in parasite and insecticide resistance in mosquito vector, malaria control has been complicated. On the other hand, due to the high price of drugs to treat malaria, Preparation by poor countries it is not possible. Therefore, prepare a safe, effective and cheap vaccine is a priority for global health. Transmission blocking vaccines (TBVs) block the completion of sexual cycle of parasite inside the mosquito or inhibit the transmission of malaria parasite from mosquitoes to humans. Invasion of the Sporozoite to salivary glands of Anopheles mosquito is a necessary step to transmission of the parasite into human body. In this stage, the interaction between Plasmodium Sporozoite TRAP Protein and the mosquito salivary glands Saglin protein happens. Down regulation of Saglin expression results in strong inhibition of salivary gland invasion. In this study, at first conserved areas Saglin gene identified and was collected data by using PCR, TA-Cloning and 3'-RACE. Molecular data analyzed by using different software. Bioinformatics analysis of the molecular data which obtained from experimental studies, indicate that Saglin and SG1B genes are ortholog in Anopheles gambiae and Anopheles stephensi. Comparison of SG1B genes Anopheles stephensi from different areas and their genetic diversities and the effect of natural selection on SG1B gene was performed in this study. Natural selection analysis determined that this gene plays an important role in the life cycle of the Anopheles mosquito. Therefore, introduced SG1B gene can be introduced as a new target for designing transmission blocking vaccine.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Malaria, Vaccine, Anophele Stephens, Saglin, SG1B, PCR, TA-Clong |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Oct 2016 06:24 |
| Last Modified: | 25 Oct 2016 06:24 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/901 |
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