Biological control of Fusarium oxysporum f.sp. lycopersici using antagonistic agents of tomato rhizosphere

Jalali, S. (2014) Biological control of Fusarium oxysporum f.sp. lycopersici using antagonistic agents of tomato rhizosphere. Masters thesis, University of Zabol.

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Tomato is one of the cosmopolitan vegetables. The quantitative and qualitative characteristics of tomato are influenced by a variety of diseases, where the plant is severely affected. Fusarium wilt is among the fungal diseases of tomato and it can become epidemic. The Disease is caused by Fusarium oxysporum (Schlechtendi: fr.) f.sp. Lycopersici (saac.) Synder and Hassen. In the research the contaminated and healthy tomato plants which was gathered from different regions of Lorestan province. The samples along the adhesive soil were transferred to the laboratory which, 15 bacterial isolates and 27 fungal isolates, were separated from crop’s rhizospher. In the next step, antagonistic effects of the isolated bacteria and fungai on F. oxysporum f.sp. Lycopersici were studied through using dual culture method. Antagonistic effects of the bacterial isolates, Bacillus subtilis and Pseudomonas fluorescens, and fungal isolates, Trichoderma harzianum and Trichoderma virens, and also biological toxin, Trichomix-H, were studied in the greenhouse. Inoculum of F. oxysporum f.sp. lycopersici in a weight proportion of 10 percent and was mixed with the pot sterilized soil to fill the upper 1/3 of the pots. The bottom 2/3 of the pots had been filled with the sterilised soil. The pots were kept for 25 days in the greenhouse and evaluation of biocontrol capacity of the antagonists in disease control was conducted simultaneously through cultivating the crop by using saturating soil method. The trial was conducted based on a completely randomized design for each antagonist with three replicates. The data were analyzed by using SPSS software and the average comparison of the traits was conducted using Duncan test. The results of the greenhouse experiments revealed that, respectively the antagonistic bacteria B. subtilize and P. fluorescence, controlled 100 and 62.5 percent the disease and The antagonistic fungi T. harzinum, T. virens, Trichomix-H50 , Trichomix-H30 controlled 100, 66.67 ,62.96 and 55.56 percent the disease.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Biological control, Fusarium wilt, Competition, Nutrient, Tomato
Subjects: Q Science > QK Botany
Depositing User: admin admin1 admin2
Date Deposited: 25 Oct 2016 06:10
Last Modified: 25 Oct 2016 06:10

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