Yardehnavi, Fatemeh (2010) Evaluation of resistance to Erwinia amylovora in apple, pear and quince and the molecular tracing of the bacterium in resistant varieties. Masters thesis, University of Zabol.
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Abstract
Fire blight caused by Erwinia amylovora (Burrill, 1882), is one of very important disease of pome fruit in Iran. The disease damages the quince, pear and apple trees in some area of country. Using resistant varieties is one of the most effective methods of disease control. In order to evaluate the resistance of 16 apple, 6 pear and 2 quince cultivars, used the Index of Varietal Susceptibility (I.V.S). By daily inspection, were written the I.V.S for each cultivar in each interval (i.e.: 5, 12, 23 and 46 days after inoculation) and also cut a branch of each cultivar randomly at 50 cm below the shoot. Results have shown only Red-Delicious, Khoje-Esfarayen, Khushe-Kharv, Oghaz- Shirvan and Morabbaei cultivars are resistant to fire blight. In next step, DNA of this resistant cultivars were extracted and a sequence of genomic DNA of bacteria were multiplied using bacteria specific primers that amplify one 1269bp fragment of bacteria genome. By running the PCR products on agarose gel, we trace the bacteria in resistant cultivars. According to the results, found that multiplication and systemic movement of the bacteria in resistant varieties occurred in different period of time. Also it showed the remaining of the latent bacterium in the distant symptomless region which can be dangerous after any damages.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Erwinia amylovora, Fire blight, Apple, Pear, Quince, Resistance, PCR |
| Subjects: | Q Science > QK Botany |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Oct 2016 06:06 |
| Last Modified: | 25 Oct 2016 06:06 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/898 |
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