Roudini, M. (2015) Effects of Bio-fertilizers on induction of cucumber resistance to damping-off disease caused by Phytophthora melonis. Masters thesis, University of Zabol.
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Abstract
One of the most common and widespread cucurbits products of the world is Cucumber which scientifically named Cucumissativus. Cucumber damping-off disease which is created by phytophthoramelonis soil Mushroom, and is a prevalent disease of cucumber. Using fungicides beside environmental problems, is not considered a sustainable method to control disease. In last half century, using biological compounds has been reported as an effective and certain method to reduce pollution to diseases. Biological fertilizers such as Glomus mosseae and Trichoderma harzianum mushrooms were utilized as biologic control factors versus phytophthora melonis mushroom-liked of cucumber bush-damping factor. Usage of the fertilizers was performed in planting Ragm Kayhan cucumber seed bed in the form of a completely random plan with three repeats. The bushes were inoculated with the disease factor in a three-leaf stage. The experiment was performed as factorial in the form of a completely random plan with three repeats and data variance were analyzed by using SPSS software and the cares mean were compared using LSD test in %5 level. The results have shown that both care had a significant difference with witness sample. In order to survey resistance molecular mechanism of cucumber seedlings against disease, Changes in expressing PAL, cupi4, Lipoxygenase, Gal’s genes were measured using QRT-PCR method. Data were analyzed by method PFFAFL and the extent of expressing considered genes were surveyed for all cares and it's measured total amount of protein, total phenol, peroxidase enzyme, polyphenol oxidase in next stage and finally, the role of fertilizer was specified in creating resistance and expressing the resistance genes. The results represented that expressing the genes have been influenced by cares and trichoderma cares had more increasing effects than other cares on expressing the genes. According to the achieved results, the fertilizers can be used as both plant boosters and bio control factors of disease.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | biological fertilizers, expressing Resistance genes, inducing resistance, cucumber bush-damping, Phytophthora melonis |
| Subjects: | S Agriculture > S Agriculture (General) |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Oct 2016 05:59 |
| Last Modified: | 25 Oct 2016 05:59 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/895 |
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