Abdi, H. (2014) Effect silica and silver nanoparticles and biological agents Trichoderma harzianum and Trichoderma viride on cucumber resistance to the pathogen Fusarium oxysporum f.sp. radiciscucumerinum. Masters thesis, University of Zabol.
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Abstract
In this research the combination of the two methods of induction of resistance and biological control was conducted by using silica and silver nanoparticles (retardants chemical induction) and T. harzianum, T. viride (agent biocontrol many of plant pathogens), Order to induce resistance in cucumber against Fusarium stem and root rot Negin in cultivars and the Festival, respectively than pathogenic agent Fusarium oxysporum f.sp. Radicis-cucumerinum resistant and susceptible are the were evaluated in the laboratory and greenhouse. The effect of petri dishes in 5, 10, 30, 50 and 100 ppm silica and silver nanoparticles by taking control in three replications (only two times sterilized containing distilled water) was conducted in order to determine effective dose range. we Investigate the effect silica nanoparticles results in preventing the growth of pathogenic fungi row of three and five days after showed that Different concentrations of silica nanoparticles in comparison with control treatments had over effect of growing colony diameter And was significant in 1% level, So that between most effective treatments to reduce the concentration of colony diameter ppm 50 disease Fusarium oxysporum f.sp. Radicis-cucumerinum 43 have; but the inhibitory effect on the growth of silver nanoparticles did not fungus, Testing was conducted in a completely randomized design in the lab. In greenhouse experiments of susceptible cultivar and resistant cultivar Festival was used Negin, The pots containing sterilized soil resistant and sensitive seed cultivars to became implanted number four seeds in each pot And at seedling stage with pathogenic fungi infected And after spending two weeks the seedlings are infected, 6-4 leaf stage by Alysytvrhay bio (T. harzianum, T. viride) and abiotic (silica nanoparticles and silver), and the interaction between living and non-living triggers both types the treatment and Induction resistance and indices, including enzymes peroxidase, polyphenol oxidase, catalase and total phenolic content of and total protein content in the tissues of treatment plants were measured. It's a completely randomized design with factorial arrangement of consists of nine original invoice (treatments applied) and five sub- plots (measured values) with three replications was conducted in greenhouse conditions. The results obtained have shown that among treatment, cultivars, days sampling and their interactions in plants treated induced resistance in comparison with the healthy control and infected plants was significant in level 5% and 1%, So that the highest level activity of phenolic compounds in resistant cultivars Festival treated with both factors antagonistic T. harzianum, T. viride with 73/0 in a maximum total protein in the Festival (tolerance) treated with silver nanoparticles 100 ppm with 123 a group and tenth was observed in the timeframe and the enzyme peroxidase, polyphenol oxidase and catalase enzyme activity the highest rate of change of the a combination treatment T. harzianum, T. viride in cultivar festival was observed incurred. The highest amounts of these compounds induce of the third day after the application has reached its to the maximum amount on the seventh day And then declined, but continues to the healthy control and infect the next on days showed significant differences.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Fusarium oxysporum, resistance, silica, silver, Trichoderma harzianum, Trichoderma viride |
| Subjects: | Q Science > QK Botany |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Oct 2016 05:56 |
| Last Modified: | 25 Oct 2016 05:56 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/894 |
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