Determination of trichothecene chemotypes of Fusarium graminearum isolates in wheats of Sistan va Baluchestan province

Mahmoodi, Payam (2012) Determination of trichothecene chemotypes of Fusarium graminearum isolates in wheats of Sistan va Baluchestan province. Masters thesis, University of Zabol.

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Fusarium head blight (FHB) is one of the most important fungal diseases of wheat in the world. Sterility of the florets, formation of shriveled seeds, and reduction in grain are characteristics of FHB which lead to yield losses in wheat. Fusarium graminearum isolates produce a large number of trichothecenes including deoxynivalenol and its derivatives, nivalenol and fusarenone x, and the estrogenic mycotoxin zearalenon. This research is about detecting the genes involved in trichothecnes production in Fusarium graminearum isolates, the causal agents of wheat Fusarium Head Blight (FHB) in Sistan and Baluchestan Province. The wheat ear samples were collected from five cities in Sistan and Baluchestan province including; Zabol, Zahedan, Saravan, KHash and Iranshahr sampling was performed in March and April, 2010. The fungal isolates were separated, purified, and identified using Fusarium keys. Totally, 293 isolates of Fusarium species belonging to nine species were isolated and identified. The isolates identified as FHB, F. graminearum have shown the highest frequency (68.8%) compared to other species. When the isolates of F. graminearum were morphologically identified, 168 isolates were chosen using species-specific primer pairs (Fg16F/Fg16R). In these isolates, the presence of three genes: Tri13, Tri5 and Tri7 were detected by using PCR and specific primers for the genes (Tri13, Tri5, and Tri7). With gene specific primer pairs, the four trichothecene chemotype including; NIV and DON was detected among isolates of F. graminearum. The results of PCR reaction with primers have shown that all isolates tested possess the genes involved in production of trichothecene. Also in the current study, two populations of 7C1 and 6A5 were identified in between F. graminearum isolates. 7C1 population (Lineage 7) was highly distributed in different regions and the dominant chemotype in this population was DON. To check the relationship of these genes and trichothecene production, six representative isolates were analyzed by HPLC. Trichothecene production was tested by HPLC in eight isolates of F. garminearum. The results confirmed the capability of trichothecene production in the studied isolates. Therefore, the detection of genes involved in trichothecene production using species-specific primers determine Fusarium isolates that produce trichothecene can alternative the chemical expensive and time procedure.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Trichothecene, chemotype, Fusarium graminearum, NIV, DON, Sistan and Baluchestan province
Subjects: Q Science > QK Botany
Depositing User: admin admin1 admin2
Date Deposited: 25 Oct 2016 05:48
Last Modified: 25 Oct 2016 05:48

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