Detection of Nivalenol and Deoxynivalenol chemotypes of wheat Fusarium culmorum isolates in West Azarbayjan province

Lava, Azad (2012) Detection of Nivalenol and Deoxynivalenol chemotypes of wheat Fusarium culmorum isolates in West Azarbayjan province. Masters thesis, University of Zabol.

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Fusarium Head Blight is known as one of the most important plant diseases in the world. This disease contaminate seeds with mycotoxins and make product decrease in quality and quantity. The main goal of the present study is to determine the produced chemotypes by the West Azarbayjan province Fusarium culmorum isolates with detection the tri7 and tri13 genes. Sampling took place during June in the crop year 2010-2011 from the farms of Urmia, Mahabad, Miandoab, Khoy, Tekab and Maku. 98 Isolates were identified as F. culmorum by conidial morphology and colony characteristic. To confirm visual identifications of F. culmorum, C51F/C51R species-specific primer pair were tested. The PCR assays results approved the results of identification based on morphological properties. To determination DON and NIV chemotypes, produced by F. culmorum isolates, Tri13 and tri7 genes were detected by primer pairs Tri13F/Tri13DONR, Tri13NIVF/Tri13NIVR, Tri7F/Tri7R and MinusTri7F/MinusTri7R. According to the PCR results, 59 isolates were introduced as DON producers and 39 isolates introduced as NIV producer. In order to survey the toxin production, 6 representative F. culmorum isolates were examined by high performance liquid chromatography. In all six isolates analyzed by this method toxin production was observed. According to the results, producing of the both DON and NIV chemotypes in west Azerbaijan province farms has been proved and the DON Chemotype was introduced as the predominant type in the reviewd farms. Molecular techniques were more accurate and rapid compared with the identification of the species by morphological characteristics and the evaluation of toxins by HPLC, also no high speciality and sophistication is needed in the use of these techniques in plant pathology. The results have shown that use of specific-specific primer pair C51F/C51R could be a proper replace for the identification of F. culmorum species based on morphological properties. The employment of the used primers in this study is highly suggested with respect to time consuming HPLC method and its high costs. Because of rather weak results obtaind by different methods for managing this disease during the prolong years, limiting of toxin synthesis has been considered as the best way of managing this disease. Accordingly detection of biochemical and molecular mechanisms and the factors adjusting the biosynthesis of the toxins, are being accounted as the basic condition for the becoming of this subject.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Deoxynivalenol, Nivalenol, Fusarium culmorum, West Azarbayjan
Subjects: Q Science > QK Botany
Depositing User: admin admin1 admin2
Date Deposited: 24 Oct 2016 07:05
Last Modified: 24 Oct 2016 07:05

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