Sahraei, SH. (2014) Isolation and cloning part of CL9P4 gene from local camel and transfer its to E.Coli to produce green vaccin. Masters thesis, University of Zabol.
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Isolation and cloning part of CL9P4 gene from local.pdf Download (218kB) | Preview |
Abstract
In recent years, with advances in molecular biology and biotechnology, research on vaccine plant has become a very important issue. There have been many benefits for vaccine production in transgenic plants such that it can be produced at low cost, ease of maintenance, lack of transmission and compatibility with the immune system mentioned above. Due to the widespread use of diagnostic and therapeutic monoclonal antibodies, production of permanent, safe and cost are important. The range of these molecules, such as enzymes to the medication; toxin for the treatment of cancer, viruses for gene therapy, and targeted liposomes for drug delivery devices for the diagnosis with certainty is important. Due to the lack of information about the presence of a monoclonal antibody-producing genes in native camel in the area and the potential diagnostic and therapeutic applications of gene CL9P4 in cancer, this study aimed to isolate and clone the gene CL9P4 camels native to the E.coli, in order to provide a suitable ground for new studies on producing green vaccines. After sample preparation and DNA extraction from blood, camel CL9P4 was designed with primers and gene amplification and direct sequencing were sent. Alignment sequencing with the results of other sequences in the NCBI made to ensure the accuracy of the amplified fragment. The gene was cloned into the E.coli CL9P4 during Laygyshn and transformation. Plasmid extraction was carried out and Bam HI digestion was done with enzymes. The results showed that the cloned gene CL9P4 in most endemic area and may exist in E.coli.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Gene CL9P4, Camel, Escherichia coli, Cloning |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Jun 2016 07:04 |
| Last Modified: | 25 Jun 2016 07:04 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/873 |
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