firoozkoohi, A. (2014) Genetic diversity and phylogenetic grouping of Escherichia coli isolates Collected from fecal samples of patients attending hospitals in Zabol. Masters thesis, University of Zabol.
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Abstract
Escherichia coli are the most common bacteria causing digestive and other extra intestinal infections in human and animals. Commensal E. coli isolates are phylogenetically distributed among geographically distinct human populations. The aim of this study was to determine the genetic diversity and phylogenetic background of commensal E. coli isolates collected from fecal samples of patients in Zabol, Iran using random amplified polymorphic DNA (RAPD) and triple-PCR respectively. A total 100 E. coli isolates were identified by conventional methods. Out of 100 E. coli isolates, 28%, 7%, 48% and 17% were fall in phylogenetic group A, B1, B2 and D respectively. The RAPD data showed a genetic similarity between 14 and 100%. In conclusion, phylogenetic group B2 and A predominated in fecal isolates, while the percentage of fecal isolates belonging to groups B1 and D was significantly lower than other.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Escherichia coli, fecal samples, genetic diversity, phylogeneticy |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Jun 2016 06:43 |
| Last Modified: | 25 Jun 2016 06:43 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/864 |
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