Hormozi, B. (2015) Frequency of virulence fimH, irP2, wcaG and rmpA genes in Klebsiella pneumoniae isolates collected from patient attending zahedan teaching hospitals. Masters thesis, University of Zabol.
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Abstract
Klebsiella pneumonia is an important nosocomial pathogen most frequently causing pneumonia, and urinary tract, wound or blood infections. These strains carry major virulence factors such as; polysaccharide capsule, adhesion, siderophore and toxins, which helps to develop infections. The first step to confront and inhibit of this pathogen is the identification of the major virulence factors. The main objective of this study was to determine the frequency of virulence factors genes encoding, capsular synthesis (wcaG), Iron acquisition systems (irp2), adhesions (fimH) and regulator of mucoid phenotype a (rmpA) using Multiplex PCR method. In this research the 16S-23S rRNA klebsialla pneumonia special marker gene was used as internal control. Among 100 Klebsiella pneumonia isolates, fimH gene with 88% and rmpA with 6% were the highest and lowest frequencies, respectively. Gene frequency for irp2 and wcaG genes were detected 87% and 33%, respectively. This study showed that genes irp2, fimH and wcaG are more abundant and important in isolates of Zahedan.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Klebsiella pneumonia, Virulence factors, Multiplex PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 25 Jun 2016 06:37 |
| Last Modified: | 25 Jun 2016 06:37 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/862 |
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