Aali, N. (2014) Investigating the effect of nicotine on nicotinic α7 receptor and Bax and Bcl-2 expression in drug resistance situation in MCF-7 breast cancer cells. Masters thesis, University of Zabol.
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Investigating the effect of nicotine on nicotinic α7 receptor and Bax and Bcl-2.pdf Download (434kB) | Preview |
Abstract
Breast cancer is a one of the main factors death in women. Nicotine is a carcinogen that causes cancer and progression of the disease. The binding of nicotine with nicotinic acetylcholine receptor (nAChR) cause stimulate cell division and increased drug resistance in cancer. Yet, effective mechanism of nicotine on breast cancer is unknown. Here, we examined nicotine effect on nicotinic α7 receptor gene, Bax, and Bcl-2 protein expression in drug resistance status in MCF-7 breast cancer cells. Main methods: To evaluate drug resistance, Human mammary gland epithelial adenocarcinomas (MCF-7) was treated with 100µL of nicotine. Then the cells were treatment with 1-5 µl/ml of doxorubicin. Cell viability was determined by MTT assay. Apoptosis biochemical parameters including Bax, Bcl-2, and α7 receptor proteins were determined by Western blotting. Nicotinic α7 receptor gene expression level was assessed by qPCR. Key findings: In this study, we demonstrated effectively signaling pathways of nicotine on nicotinic α7 receptor gene, Bax, and Bcl-2 protein expression that the growth of the breast cancer cells. Significance: The results showed (i) nicotinic α7 receptor, and Bcl-2 protein increasing but (ii) Bax protein decreasing. (iii) Nicotinic α7 receptor gene expression level was not significantly different compared with control.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Breast cancer, nicotinic α7 receptor gene, Bcl-2, Bax, apoptosis, drug resistance |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 22 Jun 2016 08:38 |
| Last Modified: | 22 Jun 2016 08:38 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/834 |
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