Koohzadi, F. (2009) Influence of TYDC Overexpression for enhancing of pharmaceutical Alkaloides in opium poppy (Papaver somniferum L.). Masters thesis, University of Zabol.
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Abstract
Plant of Papaveraceae family due to the valuable and consumed alkaloids production, including morphine, codeine, Tebaine, Noskapine, Papaverine and Sanguinarine have particular commercial importance in pharmaceutical industries. L - Tyrosine decarboxylase (TYDC) gene is starting point in pathway morphine group alkaloids in opium poppy and catalysis a reaction that resulted production Tyramine from tyrosine, and after 17 enzymatic stages at least, banzene ring changes into codeine and morphine. The purpose of this study is overexpression of TYDC2 gene in accumulation of Benzilizoquinoline alkaloids and in the following increasing synthesis of morphine group alkaloids. For achieve to this target PBI121-TK recombinant construct was built with TYDC containing Kozak sequence in region of Gus in pBI121 plasmid under the CaMV35S promoter and Nos terminator. The gene cloning results was confirmed by using different methods, molecular enzymatic digestion, PCR method and histoc-himical Gus test. HPLC results of transgenic plant with agroinfiltration method showed that the amount of alkaloids in these plants was significantly increased than no transgenic plants.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Benzilizoquinoline Alkaloides, Tyrosine decarboxylase (TYDC), Gene Overexpression, Papaver somniferum, Agro infiltration |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 21 Jun 2016 08:23 |
| Last Modified: | 21 Jun 2016 08:23 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/824 |
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