Vafadarnejad, Akram (2015) type I - V SCCmec in Staphylococcus isolated from clinical samples in the city of Zabol by Multiplex PCR. Masters thesis, University of Zabol.
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Identification of type I - V SCCmec in Staphylococcus aureus isolated.pdf Download (73kB) | Preview |
Abstract
Staphylococcus aureus is one of the main causes of nosocomial and community-acquired infections. Methicillin - resistant Staphylococcus aureus strains (MRSA) is the most important cause of nosocomial infections. Resistance to methicillin causes by the mecA gene encoding protein (PBP2a) ، the gene is located in staphylococcal chromosome cassette (SCCMEC). Typing of this region is essential for understanding transduction pathways and genetic studies of the bacteria. Moreover precise study of Staphylococcus aureus in genetic level is essential for understanding of the involved mechanisms in resistance. The main objective of this study was to identify the types I - V SCCmec in clinical Staphylococcus aureus isolates from the city of Zabol by multiplex-PCR method. After DNA extraction by boiling method and multiplex-PCR using specific primers, SCCMEC genes were identified in these bacteria. Results showed 5% type I, 45% type II, 30% Type III and Type V 20% among the 100 Staphylococcus aureus isolates, and SCCmec II was dominant in the Staphylococcus aureus isolates of Sistan hospitals.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Methicillin-resistant Staphylococcus aureus (MRSA), PBP2a, staphylococcal cassette chromosome (SCCMEC) |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 20 Jun 2016 07:54 |
| Last Modified: | 20 Jun 2016 07:54 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/808 |
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