HER2 proto-oncogene promoter methylation status study in breast cancer

Hosseinzadeh, Leila (2013) HER2 proto-oncogene promoter methylation status study in breast cancer. Masters thesis, University of Zabol.

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Breast cancer is one of the most common cancer kinds among women and the second leading cause of death after lung cancer. It is include 32% of all kinds of cancers among women. Beside several known genetic alterations, several epigenetic alterations have been discovered that are involved in the process of tumor genesis in breast tissue and lead to sustainable change in patterns of gene expression in cells and can cause cancer progression. HER2 proto-oncogene encoding a protein that targets an intracellular signaling pathways and plays an important role in growth, differentiation, survival and cell adhesion. This gene is inactivated by genetic and epigenetic different mechanisms such as hyper methylation. The purpose of this study is to evaluate the methylation of promoter gene HER2 and to determine its possible association with prognostic factors in the studied patients. 40 samples of blood and breast tissue of patients with invasive ductal carcinoma and 10 samples of blood and normal tissues of healthy people were collected from those who came to the cancer center academic Jahad. After DNA extraction from blood and tissue samples, concentrations were determined by spectrophotometry and then appropriate samples were placed in freezer -70 oc. The methylation status of the target gene was assessed using Methylation Specific PCR method. After electrophoresis, data was analyzed by using software SPSS ver 19. Ficher Exact Test and Chi square, for two-state variables and Kruskal Wallis Test and Chi square, for three-state variables with a significance level of p>0.05 were used. Following the study, 30% of tissue samples and 30% of blood samples were hyper methylated. Evolution results showed no significantly association between HER2 hyper methylation and studied factors. But as expected, HER2 hyper methylation was significantly associated (p=0.03) with the absence of HER2 gene expression as a prognostic factor, that indicated the greatest role of methylation in the inactivation of this gene in Iranian patients.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Breast cancer, methylation, HER2 gene
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: admin admin1 admin2
Date Deposited: 20 Jun 2016 07:04
Last Modified: 20 Jun 2016 07:04
URI: http://eprints.uoz.ac.ir/id/eprint/800

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