Genetic variation assessment of Sistan wheat cultivars using AP-PCR method

Mehrabi nejad, E. (2012) Genetic variation assessment of Sistan wheat cultivars using AP-PCR method. Masters thesis, University of Zabol.

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Abstract

Polymorphisms in genomic fingerprints generated Byarbitrarily primed PCR (APPCR) can distinguish between strains of almost any organism. To investigate the AP-PCR method, 20 cultivars of Sistan wheat collected and studied using AP-PCR method. Fourteen-days-old plants from pots were used to extract DNA. Four pairs of specific primers used STS method to AP-PCR. The observed polymorphism was analyzed by multivariate methods. Six principal coordinates explained 64.161% of the variation among the studied population. Cultivars were divided into 11 groups, at the level of 0.55 using Jaccard’s similarity coefficient. Results indicated that The AP-PCR category Issome what more accurate than the STS method.

Item Type: Thesis (Masters)
Uncontrolled Keywords: AP-PCR, genetic distance, Sistan wheat
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 12 Jun 2016 05:30
Last Modified: 12 Jun 2016 05:30
URI: http://eprints.uoz.ac.ir/id/eprint/751

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