Kazemi Sani, R. (2010) Evaluation of GSK gene expression in selected wheat Lines using Real Time PCR. Masters thesis, University of Zabol.
|
Text
Evaluation of GSK gene expression in selected wheat.pdf Download (90kB) | Preview |
Abstract
In order to determine TaGSK1 gene expression levels in 25 selected wheat genotypes, a comparison between genotypes were performed under control and stress conditions in Zabol University Biotechnology Institute. Three replicates for each genotype was considered and then specimens under stress were subjected NaCl 200 mM. RNA was extracted from leaf samples. cDNA was designed using reverse transcription. 18S rRNA gene expression was used as internal control gene in Real Time PCR to normalize TaGSK1 gene expression levels. Results showed Sistan genotype had significant differences between level of TaGSK1 gene expression under conditions of salinity and control. In this genotype, TaGSK1 gene expression showed maximum increased to 2.5 fold and lowest increased to 1.1 fold observed in S-82-15 genotype.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | TaGSK1 gene, Gene expression, Salt stress, Wheat and Real Time PCR |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 05 Jun 2016 05:16 |
| Last Modified: | 05 Jun 2016 05:16 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/715 |
Actions (login required)
 |
View Item |
