Pourrahmat, E. (2013) Evaluation of epidermal growth factor receptor gene Expression in patient whit colorectal cancer in Sistan and Baluchistan province by reverse transcriptase real-time Polymerase chain reaction. Masters thesis, University of Zabol.
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Evaluation of epidermal growth factor receptor gene expression in patient whit colorectal cancer in Sistan.pdf Download (171kB) | Preview |
Abstract
Colorectal cancer is the third most common cause of death in the world also third and fourth most common cancer among men and women respectively in Iran. Epidermal growth factor receptor is a tyrosine kinas receptor that shows over expression in epithelial tumors and regulates important processes in tumor genesis. Differences in genetic susceptibility and exposure to a variety of environmental and nutritional factors in the incidence and characteristics of colorectal cancer are based on the geographic region and race. In the present study, the expression of EGFR in formalin fixed paraffin-embedded (FFPE) colorectal cancer tissue samples in the province of Sistan and Baluchistan of Iran were studied. Fifteen FFPE colorectal cancer tissue samples from medical centers in Sistan and Baluchistan of Iran were analyzed for EGFR gene expression level by real-time quantitative reverse transcriptase polymerase chain reaction. All PCR reactions were performed in triplicate for both target gene and internal control (18s rRNA) with the 2-00Ct method. Differences in target gene expression levels in patients and controls were evaluated with t-test. P ≤ 0.05 was considered to be significant. All analyses were performed using the SPSS 13 software (SPSS, Inc., Chicago, IL). The results showed EGFR gene over expression in 12 (80%) of 15 patients. There was a statistically significant difference in the prevalence of EGFR expression between patients and control (p<0.05). Our results demonstrated EGFR gene over expression in formalin fixed paraffin embedded (FFPE) colorectal cancer tissue samples compared with controls.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Colorectal cancer, epidermal growth factor receptor, reverse transcriptase real-time polymerase chain reaction |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 05 Jun 2016 04:53 |
| Last Modified: | 05 Jun 2016 04:53 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/710 |
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