Azizi, A. (2014) Isolation and cloning part of NbBruc02 gene from local camel and transfer its to E. Coli to produce green vaccin. Masters thesis, University of Zabol.
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Abstract
Advances in molecular biology techniques in the 1980s, has performed a large role in the development of new strategies for the production of a vaccine. Production of transgenic plants promising new vaccine production which is cheaper, safer, and more comfortable with established power storage. For this reason, plants as an important alternative method for producing a wide range of valuable active proteins were expressed in the health industry. Camel is a unique phenotypic characteristic that exist in other mammals. The unique features of the cellular and molecular aspects can also be noteworthy. NbBruc02, 03 are genes that camels are native to detect. Brucella antigens of the two main species of Brucella (B.abortus and B.melitensis) and Yersinia are the kinds that were detected. This study aimed to isolate and clone partial of NbBruc02 gene local camel and alignment and the other sequences that is available and transferring them to E.Coli bacteria were performed to produce green vaccines. Single-humped camel blood samples from a further 5-year local control were prepared in the Zabol city. DNA extraction was performed to ensure the quality and quantity of extracted DNA using spectrophotometry and agars gel. The amplified fragment of PCR temperature gradient method using specific primers was used and the junction temperature was obtained. Then Gel extraction was performed and the obtained product was sent for sequencing company. Sequence received from the company compared with sequences in the NCBI and was confirmed by Praimer BLAST software. To clone the gene of interest from a T/A vector was used. Then the plasmid was transferred into cells for E.Coli. The white colonies were sub cultured. Plasmid extraction was performed. Then the digestion of recombinant plasmids T/A was performed using the enzyme BamHI. The results showed that there are NbBruc02 gene in local camel areas and clones it successfully in E.Coli bacteria.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | NbBruc02 gene, camel, E.Coli bacteria |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 29 May 2016 09:36 |
| Last Modified: | 29 May 2016 09:36 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/705 |
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