Evaluation of Astrocyte elevated gene -1 expression in Patient whit esophageal cancer by RT-qPCR

Hashemi, Z. (2014) Evaluation of Astrocyte elevated gene -1 expression in Patient whit esophageal cancer by RT-qPCR. Masters thesis, University of Zabol.

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Esophageal cancer is one of the most invasive types of gastrointestinal cancer and the most common cause of cancer-related deaths in the world. Most of esophageal cancers occur in the age group 70-50 years. The frequency of this disease is more in men than women. Astrocyte elevated gene -1(AEG-1, also known as MTDH, and Lyric) has been demonstrated to play a potential role in several significant aspects of tumor progression. Expression or genomic amplification this gene can serve as a biological marker to identify subgroups of patients who require more advanced treatments targeting AEG-1 and benefits are used. In the present study, the expressions of AEG-1 in formalin fixed paraffin-embedded (FFPE) Esophageal cancer tissue samples in the Iran were studied. Fifteen FFPE Esophageal cancer tissue samples from medical centers were analyzed for AEG-1 gene expression level by real-time quantitative reverse transcriptase polymerase chain reaction. All PCR reactions were performed in triplicate for both target gene (AEG-1) and internal control (B-actin) with the 2- ؤؤCt method. Differences in target gene expression levels in patients and controls were evaluated with t-test. P ،ـ 0.05 was considered to be significant. All analyses were performed using the SPSS 13 software (SPSS, Inc., Chicago, IL). The results showed AEG-1 gene over expression. There was a statistically significant difference in the prevalence of AEG-1 expression between patients and control (p<0.05). Our results demonstrated AEG-1 gene over expression in formalin fixed paraffin embedded ( FFPE) esophageal cancer tissue samples compared with controls.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Esophageal cancer, AEG-1, ، Reverse transcriptase real-time polymerase chain reaction
Subjects: Q Science > QH Natural history > QH426 Genetics
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Depositing User: admin admin1 admin2
Date Deposited: 14 May 2016 09:32
Last Modified: 14 May 2016 09:32
URI: http://eprints.uoz.ac.ir/id/eprint/594

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